SIRT6 Recombinant Rabbit Monoclonal Antibody [PSH10-98]
cat.: HA723265
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH10-98 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SIRT6 aa 1-355. |
| Positive control: | HeLa cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate, HCT 116, human testis tissue. |
| Subcellular location: | Nucleus, Chromosome, telomere, Endoplasmic reticulum. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q8N6T7 Human | P59941 Mouse Entrez Gene: 299638 Rat |
| Alternative names: | 2810449N18Rik AI043036 Mono ADP ribosyltransferase sirtuin 6 NAD-dependent protein deacetylase sirtuin-6 Regulatory protein SIR2 homolog 6 Regulatory protein SIR2 homolog SIR2 like 6 SIR2 like protein 6 Sir2 related protein type 6 SIR2-like protein 6 SIR2L6 SIR6_HUMAN SIRT 6 SIRT6 Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) Sirtuin 6 Sirtuin type 6 Sirtuin6 |
Images
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Fig1:
Western blot analysis of SIRT6 on different lysates with Rabbit anti-SIRT6 antibody (HA723265) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: MEF cell lysate Lane 5: C6 cell lysate Lane 6: PC-12 cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 36/42 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723265) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HCT 116 cells labeling SIRT6 with Rabbit anti-SIRT6 antibody (HA723265) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIRT6 antibody (HA723265) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SIRT6 antibody (HA723265) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723265) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.