SIRT6 Recombinant Rabbit Monoclonal Antibody [PSH10-99]
cat.: HA723266
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH10-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SIRT6 aa 1-355. |
| Positive control: | HeLa cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate, mouse testis tissue. |
| Subcellular location: | Nucleus, Chromosome, telomere, Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q8N6T7 Human | P59941 Mouse Entrez Gene: 299638 Rat |
| Alternative names: | 2810449N18Rik AI043036 Mono ADP ribosyltransferase sirtuin 6 NAD-dependent protein deacetylase sirtuin-6 Regulatory protein SIR2 homolog 6 Regulatory protein SIR2 homolog SIR2 like 6 SIR2 like protein 6 Sir2 related protein type 6 SIR2-like protein 6 SIR2L6 SIR6_HUMAN SIRT 6 SIRT6 Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) Sirtuin 6 Sirtuin type 6 Sirtuin6 |
Images
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Fig1:
Western blot analysis of SIRT6 on different lysates with Rabbit anti-SIRT6 antibody (HA723266) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HCT 116 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: MEF cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (20 µg/Lane) Lane 7: COS-1 cell lysate (20 µg/Lane) Predicted band size: 39 kDa Observed band size: 36/42 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723266) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SIRT6 antibody (HA723266) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723266) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Western blot analysis of SIRT6 on different lysates with Rabbit anti-SIRT6 antibody (HA723266) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-SIRT6 KO cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723266) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.