ROR alpha / RORA Recombinant Rabbit Monoclonal Antibody [PSH11-00]
cat.: HA723267
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | PSH11-00 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse RORA aa aa 101-523. |
| Positive control: | Mouse cerebellum tissue, Rat cerebellum tissue, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P WB |
1:1,000 1:2,000 |
| Uniprot #: | SwissProt: P51448 Mouse Entrez Gene: 300807 Rat |
| Alternative names: | fhl2a NR1F1 Nuclear receptor ROR alpha Nuclear receptor ROR-alpha Nuclear receptor RZR-alpha Nuclear receptor subfamily 1 group F member 1 RAR related orphan receptor A RAR related orphan receptor alpha RAR-related orphan receptor A Retinoid-related orphan receptor-alpha Rora RORA_HUMAN RZR alpha RZR-ALPHA RZRA Transcription factor RZR alpha |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-ROR alpha / RORA antibody (HA723267) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723267) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-ROR alpha / RORA antibody (HA723267) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723267) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Western blot analysis of ROR alpha / RORA on different lysates with Rabbit anti-ROR alpha / RORA antibody (HA723267) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (40 µg/Lane) Lane 2: Mouse cerebellum tissue lysate (40 µg/Lane) Lane 3: Mouse spleen tissue lysate (negative) (40 µg/Lane) Lane 4: Rat cerebellum tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 59 kDa Observed band size: 56/59 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723267) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.