Human CD47 Recombinant Rabbit Monoclonal Antibody [PSH11-05]
cat.: HA723272
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH11-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD47 aa 19-141 (HA211050). |
| Positive control: | Recombinant Human CD47 protein (HA211050). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-06] to Human CD47 antibody (Detector) (HA723273) and Recombinant Human CD47 protein (HA211050) as the standard. The reference range value is 39-10.000 pg/ml. |
| Uniprot #: | SwissProt: Q08722 Human |
| Alternative names: | Antigen identified by monoclonal 1D8 Antigenic surface determinant protein OA3 CD 47 CD47 CD47 antigen (Rh-related antigen, integrin-associated signal transducer) CD47 antigen CD47 glycoprotein CD47 molecule CD47_HUMAN IAP Integrin Associated Protein Integrin associated signal transducer Integrin-associated protein Leukocyte surface antigen CD47 MER 6 MER6 OA 3 OA3 OTTHUMP00000041152 OTTHUMP00000041153 Protein MER6 Rh related antigen Surface antigen identified by monoclonal 1D8 |
Images
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Fig1:
Sandwich ELISA analysis of human CD47 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723272) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD47 protein (HA211050) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723273, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CD47 in Jurkat and HepG2 extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CD47 was measured in duplicate at different sample concentrations and interpolated from the CD47 standard curves. The mean CD47 concentration was determined to be 1,853 pg/mL in Jurkat cell extract, There was no detectable signal in HepG2 cell extract. |
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Fig3:
Interpolated concentrations of spiked CD47 in cell culture media samples. The concentrations of CD47 were measured in duplicates, interpolated from the CD47 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.