Human NLRP3 Recombinant Rabbit Monoclonal Antibody [PSH11-07]
cat.: HA723275
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH11-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NLRP3 aa 1-218. |
| Positive control: | Recombinant Human NLRP3 protein. |
| Subcellular location: | Cytoplasm, Inflammasome, Secreted, Nucleus, Endoplasmic reticulum. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-08] to Human NLRP3 antibody (Detector) (HA723276) or Rabbit monoclonal [PSH11-09] to Human NLRP3 antibody (Detector) (HA723278) and Recombinant Human NLRP3 protein as the standard. The reference range value is 78-10,000 pg/ml. |
| Uniprot #: | SwissProt: Q96P20 Human |
| Alternative names: | AGTAVPRL AII/AVP Angiotensin/vasopressin receptor AII/AVP like Angiotensin/vasopressin receptor AII/AVP-like C1orf7 Caterpiller protein 1.1 CIAS 1 CIAS1 CLR1.1 Cold autoinflammatory syndrome 1 Cold autoinflammatory syndrome 1 protein Cryopyrin Familial cold autoinflammatory syndrome FCAS FCU LRR and PYD domains-containing protein 3 Muckle-Wells syndrome MWS NACHT NACHT LRR and PYD containing protein 3 NALP 3 NALP3 NALP3_HUMAN NLRP3 PYPAF 1 PYPAF1 PYRIN containing APAF1 like protein 1 PYRIN-containing APAF1-like protein 1 |
Images
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Fig1:
Sandwich ELISA analysis of human NLRP3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723275) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NLRP3 protein starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723276, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native NLRP3 in THP-1 and Jurkat extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NLRP3 was measured in duplicate at different sample concentrations and interpolated from the NLRP3 standard curves. The mean NLRP3 concentration was determined to be 691 pg/mL in THP-1 cell extract. There was no detectable signal in Jurkat cell extract. |
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Fig3:
Interpolated concentrations of spiked NLRP3 in cell culture media samples. The concentrations of NLRP3 were measured in duplicates, interpolated from the NLRP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
|
Fig4:
Sandwich ELISA analysis of human NLRP3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723275) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NLRP3 protein starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723278, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig5:
Interpolated concentrations of native NLRP3 in THP-1 and Jurkat extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NLRP3 was measured in duplicate at different sample concentrations and interpolated from the NLRP3 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NLRP3 concentration was determined to be 888 pg/mL in THP-1 cell extract. There was no detectable signal in Jurkat cell extract. |
|
Fig6:
Interpolated concentrations of spiked NLRP3 in cell culture media samples. The concentrations of NLRP3 were measured in duplicates, interpolated from the NLRP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.