Biotin Conjugated cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH06-71]
cat.: HA723283B
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: ELISA(Det), ELISA
Clonality: Monoclonal
Clone number: PSH06-71
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Recombinant protein within human cIAP1 aa 201-618.
Positive control: Recombinant Human cIAP1 protein.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  ELISA(Det)
  ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH06-72] to Human cIAP1 antibody (Capture) (HA723280) or Rabbit monoclonal [PSH02-10] to Human cIAP1 antibody (Capture) (HA723281) and Recombinant Human cIAP1 protein as the standard. The reference range value is 62.5-8,000 pg/ml.
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q13490 Human | Q62210 Mouse
Entrez Gene: 60371 Rat
Alternative names: API 1 API1 Apoptosis inhibitor 1 Baculoviral IAP repeat containing 2 Baculoviral IAP repeat containing protein 2 Baculoviral IAP repeat-containing protein 2 BIRC 2 BIRC2 BIRC2_HUMAN C IAP1 C-IAP1 Cellular inhibitor of apoptosis 1 cellular inhibitor of apoptosis protein 1 cIAP 1 cIAP1 HIAP 2 HIAP-2 HIAP2 IAP 2 IAP homolog B IAP-2 IAP2 Inhibitor of apoptosis protein 2 MIHB NFR2 TRAF signalling complex protein RING finger protein 48 RNF 48 RNF48 TNFR2 TRAF signaling complex protein 2 TNFR2-TRAF-signaling complex protein 2
Images
HA723283B_1.png Fig1: Sandwich ELISA analysis of human cIAP1 matched pair antibodies

Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723280) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human cIAP1 protein starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723283B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723283B_2.png Fig2: Sandwich ELISA analysis of human cIAP1 matched pair antibodies

Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723281) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human cIAP1 protein starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723283B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723283B_3.png Fig3: Interpolated concentrations of native cIAP1 in THP-1 and TF-1 extract samples based on a 1,000 µg/ml extract load.

The concentrations of cIAP1 were measured in duplicates, interpolated from the cIAP1 standard curve and corrected for sample dilution. Undiluted samples are THP-1 extract 25% and TF-1 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean cIAP1 concentration was determined to be 1,712 pg/ml in THP-1 extract and 1,800 pg/ml in TF-1 extract.
HA723283B_4.png Fig4: Interpolated concentrations of native cIAP1 in THP-1 and TF-1 extract samples based on a 1,000 µg/mL extract load.

The concentrations of cIAP1 were interpolated from the cIAP1 standard curves and corrected for sample dilution. Undiluted samples are THP-1 extract 50% and TF-1 extract 50%. The mean cIAP1 concentration was determined to be 1,450 pg/ml in THP-1 extract and 2,235 pg/ml in TF-1 extract.
HA723283B_5.png Fig5: Interpolated concentrations of spiked cIAP1 in human cell culture media samples.

The concentrations of cIAP1 were measured in triplicates, interpolated from the cIAP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).
HA723283B_6.png Fig6: Interpolated concentrations of spiked cIAP1 in human cell culture media samples.

The concentrations of cIAP1 were interpolated from the cIAP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.