HRP Conjugated Human Mesothelin Recombinant Rabbit Monoclonal Antibody [PSH0-57]
cat.: HA723289H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH0-57 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Mesothelin aa 531-580 / 622 (Q13421-3). |
| Positive control: | Recombinant Human Mesothelin protein (HA210521). |
| Subcellular location: | Cell membrane, Golgi apparatus, Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH01-26] to Human Mesothelin antibody (Capture) (HA723287) and Recombinant Human Mesothelin protein (HA210521) as the standard. The reference range value is 937.5-30,000 pg/ml. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q13421 Human |
| Alternative names: | CAK 1 CAK1 CAK1 antigen cleaved form Megakaryocyte potentiating factor Mesothelin Mesothelin isoform 1 precursor MPF Msln MSLN_HUMAN Pre pro megakaryocyte potentiating factor Pre-pro-megakaryocyte-potentiating factor SMR SMRP Soluble MPF mesothelin related protein |
Images
|
Fig1:
Sandwich ELISA analysis of Human Mesothelin matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723287) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Mesothelin protein (HA210521) starting from 30,000 pg/ml to 0 pg/ml and detect antibody (HA723289H, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.