C9orf72 Recombinant Rabbit Monoclonal Antibody [PSH11-11]
cat.: HA723290
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: PSH11-11
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Recombinant protein within human C9orf72 aa 1-250.
Positive control: SH-SY5Y cell lysate, Neuro-2a cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: Q96LT7 Human | Q6DFW0 Mouse | Q66HC3 Rat
Alternative names: ALSFTD C9orf72 chromosome 9 open reading frame 72 CI072_HUMAN FTDALS MGC23980 Protein C9orf72 RP11-27J8.2 Uncharacterized protein C9orf72
Images
HA723290_1.jpg Fig1: Western blot analysis of C9orf72 on different lysates with Rabbit anti-C9orf72 antibody (HA723290) at 1/5,000 dilution.

Lane 1: SH-SY5Y cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: Human brain tissue lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723290) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.