Human MMP13 Recombinant Rabbit Monoclonal Antibody [PSH11-14]
cat.: HA723294
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH11-14 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MMP-13 aa 20-471. |
| Positive control: | Recombinant Human MMP13 protein (HA210854). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-13] to Human MMP13 antibody (Capture) (HA723293) and Recombinant Human MMP13 protein (HA210854) as the standard. The reference range value is 78-5,000 pg/ml. |
| Uniprot #: | SwissProt: P45452 Human |
| Alternative names: | CLG 3 CLG3 Collagenase 3 Collagenase3 MANDP1 Matrix metallopeptidase 13 (collagenase 3) Matrix Metalloproteinase 13 Matrix metalloproteinase-13 MMP 13 MMP-13 Mmp13 MMP13_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of human MMP13 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723293) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MMP13 protein (HA210854) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723294, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.