Phospho-MEK3 (S189)+MEK6 (S207) Recombinant Rabbit Monoclonal Antibody [PSH11-17]
cat.: HA723299
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Monoclonal
Clone number: PSH11-17
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 39/37 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser189 of Human MEK3 (P46734-2).
Positive control: HeLa cell lysate, HeLa treated with 25μg/mL Anisomycin for 30 minutes cell lysate, NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate.
Subcellular location: Cytoplasm, cytosol, membrane, nucleoplasm; Nucleus, Cytoplasm, cytoskeleton.
Recommended Dilutions:
  WB

1:15,000
Uniprot #: SwissProt: P46734 Human | P52564 Human | O09110 Mouse | P70236 Mouse
Entrez Gene: 114495 Rat | 303200 Rat
Alternative names: Dual specificity mitogen activated protein kinase kinase 6 Dual specificity mitogen-activated protein kinase kinase 3 MAP kinase kinase 3 MAP kinase kinase 6 map2k3 MAP2K6 MAPK/ERK kinase 3 MAPK/ERK kinase 6 MAPKK 3 MAPKK 6 MAPKK3 MAPKK6 MEK 3 MEK 6 Mitogen activated protein kinase kinase 3 Mitogen activated protein kinase kinase 6 MKK3 MKK6 MP2K3_HUMAN PRKMK3 PRKMK6 Protein kinase, mitogen activated, kinase 6 (MAP kinase kinase 6) SAPK kinase 2 SAPKK 3 SAPKK-2 SAPKK2 SAPKK3 Stress activated protein kinase kinase 3 Stress-activated protein kinase kinase 2
Images
HA723299_1.jpg Fig1: Western blot analysis of Phospho-MEK3 (S189)+MEK6 (S207) on different lysates with Rabbit anti-Phospho-MEK3 (S189)+MEK6 (S207) antibody (HA723299) at 1/15,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 25μg/mL Anisomycin for 30 minutes cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 39/37 kDa
Observed band size: 39/37 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723299) at 1/15,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.