Presenilin 2 / AD5 Recombinant Rabbit Monoclonal Antibody [PSH11-18]
cat.: HA723300
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH11-18 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Presenilin 2 aa 301-350. |
| Positive control: | HeLa cell lysate, SH-SY5Y cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, PC-12. |
| Subcellular location: | Endoplasmic reticulum membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
WB IF-Cell FC IP |
1:2,000 1:100 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P49810 Human | Q61144 Mouse | O88777 Rat |
| Alternative names: | AD3L AD3LP AD4 AD5 Alzheimer disease 4 CMD1V E5-1 OTTHUMP00000035671 OTTHUMP00000035672 OTTHUMP00000228286 OTTHUMP00000228288 Presenilin 2 (Alzheimer disease 4) Presenilin 2 Presenilin-2 CTF subunit PS-2 PS2 Psen2 PSN2_HUMAN PSNL2 STM-2 STM2 |
Images
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Fig1:
Western blot analysis of Presenilin 2 / AD5 on different lysates with Rabbit anti-Presenilin 2 / AD5 antibody (HA723300) at 1/2,000 dilution. Lane 1: HeLa cell lysate (no heat) Lane 2: SH-SY5Y cell lysate (no heat) Lane 3: RAW264.7 cell lysate (no heat) Lane 4: Neuro-2a cell lysate (no heat) Lane 5: PC-12 cell lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 23 kDa Exposure time: Lane 1-4: 3 minutes; Lane 3: 1 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723300) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of PC-12 cells labeling Presenilin 2 / AD5 with Rabbit anti-Presenilin 2 / AD5 antibody (HA723300) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Presenilin 2 / AD5 antibody (HA723300) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of PC-12 cells labeling Presenilin 2 / AD5. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723300, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Presenilin 2 / AD5 was immunoprecipitated from 0.2 mg SH-SY5Y cell lysate with HA723300 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723300 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/10,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y cell lysate (input) Lane 2: HA723300 IP in SH-SY5Y cell lysate Lane 3: Rabbit IgG instead of HA723300 in SH-SY5Y cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.