CYLD Recombinant Rabbit Monoclonal Antibody [PSH11-21]
cat.: HA723303
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH11-21 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 107 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CYLD aa 1-250. |
| Positive control: | 293T cell lysate, HeLa cell lysate, Jurkat cell lysate, COS-1 cell lysate, Mouse brain tissue lysate, Jurkat, RAW264.7, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cytoplasm, perinuclear region, cytoskeleton, Cell membrane, microtubule organizing center, centrosome, spindle, cilium basal body. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:2,000 1:1,000 1:100 |
| Uniprot #: | SwissProt: Q9NQC7 Human | Q80TQ2 Mouse | Q66H62 Rat |
| Alternative names: | BRSS CDMT Cyld CYLD gene CYLD_HUMAN CYLD1 CYLDI cylindromatosis (turban tumor syndrome) cylindromatosis 1 Deubiquitinating enzyme CYLD EAC HSPC057 KIAA0849 MFT MFT1 Probable ubiquitin carboxyl terminal hydrolase CYLD SBS TEM turban tumor syndrome Ubiquitin carboxyl-terminal hydrolase CYLD ubiquitin specific peptidase like 2 ubiquitin thioesterase CYLD Ubiquitin thiolesterase CYLD Ubiquitin-specific processing protease CYLD Ubiquitin-specific-processing protease CYLD USPL2 |
Images
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Fig1:
Western blot analysis of CYLD on different lysates with Rabbit anti-CYLD antibody (HA723303) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: COS-1 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse brain-CYLD KO tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 107 kDa Observed band size: 107 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723303) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Jurkat cells labeling CYLD with Rabbit anti-CYLD antibody (HA723303) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CYLD antibody (HA723303) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of RAW264.7 cells labeling CYLD with Rabbit anti-CYLD antibody (HA723303) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CYLD antibody (HA723303) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CYLD antibody (HA723303) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723303) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-CYLD antibody (HA723303) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723303) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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