SMOC2 Recombinant Rabbit Monoclonal Antibody [PSH11-25]
cat.: HA723307
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH11-25 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse SMOC2 aa 1-447. |
| Positive control: | NIH/3T3 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human liver cancer tissue. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix, basement membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200 |
| Uniprot #: | SwissProt: Q9H3U7 Human | Q8CD91 Mouse Entrez Gene: 292401 Rat |
| Alternative names: | bA270C4A.1 bA37D8.1 (secreted modular calcium binding protein 2) bA37D8.1 dJ421D16.1 (novel thyroglobulin type 1 repeat containing protein) dJ421D16.1 MST117 MSTP117 MSTP140 Secreted modular calcium binding protein 2 Secreted modular calcium-binding protein 2 SMAP 2 SMAP-2 SMAP2 SMOC 2 SMOC-2 SMOC2 SMOC2_HUMAN Smooth muscle associated protein 2 Smooth muscle-associated protein 2 SPARC related modular calcium binding 2 SPARC-related modular calcium-binding protein 2 |
Images
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Fig1:
Western blot analysis of SMOC2 on different lysates with Rabbit anti-SMOC2 antibody (HA723307) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723307) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-SMOC2 antibody (HA723307) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723307) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.