Lipoprotein a Recombinant Rabbit Monoclonal Antibody [PSH11-29]
cat.: HA723311
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH11-29 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 227 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human LPA aa 1-1,050. |
| Positive control: | Human serum lysate, Human plasma lysate, human liver tissue, human glioma tumor tissue. |
| Subcellular location: | Extracellular region, extracellular space, plasma lipoprotein particle. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:2,000 1:1,000 1:200 |
| Uniprot #: | SwissProt: P08519 Human |
| Alternative names: | AK38 Antiangiogenic AK38 protein apo(a) APOA apolipoprotein(a) LP lp(a) LPA Lipoprotein a |
Images
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Fig1:
Western blot analysis of Lipoprotein a on different lysates with Rabbit anti-Lipoprotein a antibody (HA723311) at 1/2,000 dilution. Lane 1: Human serum lysate Lane 2: Human plasma lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 227 kDa Observed band size: 500 kDa Exposure time: 10 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723311) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Lipoprotein a antibody (HA723311) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723311) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human glioma tumor tissue with Rabbit anti-Lipoprotein a antibody (HA723311) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723311) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human brain tissue (negative) with Rabbit anti-Lipoprotein a antibody (HA723311) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723311) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.