iFluor™ 647 Conjugated Estrogen Receptor Recombinant Rabbit Monoclonal Antibody [PD00-04]
cat.: HA723312F
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: PD00-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 66 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Estrogen Receptor alpha aa 300-595 / 595.
Positive control: Human breast cancer tissue.
Subcellular location: Cell membrane, Nucleus, Cytoplasm.
Recommended Dilutions:
  IF-Cell
  IF-Tissue

1:100
1:200
Uniprot #: SwissProt: P03372 Human
Alternative names: 7*/654 isoform 7*/819 2 isoform 7*/822 isoform 8*/901 isoform 8*/941 isoform DKFZp686N23123 ER alpha ER ER-alpha Era ESR ESR1 ESR1_HUMAN ESRA Estradiol receptor Estrogen nuclear receptor alpha Estrogen receptor 1 Estrogen receptor alpha 3*,4,5,6,7*/822 isoform Estrogen receptor alpha Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform Estrogen receptor alpha delta 4 +49 isoform Estrogen receptor alpha delta 4*,5,6,7*/654 isoform Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform Estrogen receptor alpha E1 E2 1 2 Estrogen receptor alpha E1 N2 E2 1 2 Estrogen receptor ESTRR NR3A1 Nuclear receptor subfamily 3 group A member 1
Images
HA723312F_1.jpg Fig1: Immunofluorescence analysis of paraffin-embedded human breast cancer tissue labeling Estrogen Receptor with Rabbit anti-Estrogen Receptor antibody (HA723312F, iFluor™ 647) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723312F, iFluor™ 647, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.