Human CXCL6/GCP-2 Recombinant Rabbit Monoclonal Antibody [PSH11-31]
cat.: HA723315
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH11-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CXCL6/GCP-2 aa 38-114 (HA211111). |
| Positive control: | Recombinant Human CTGF protein (HA211111). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-30] to Human CXCL6/GCP-2 antibody (Capture) (HA723314) and Recombinant Human CXCL6/GCP-2 protein (HA211111) as the standard. The reference range value is 15.6-2,000 pg/mL. |
| Uniprot #: | SwissProt: P80162 Human |
| Alternative names: | C X C motif chemokine 6 Chemokine (C X C motif) ligand 6 Chemokine alpha 3 CKA 3 CKA-3 CKA3 CXCL 6 CXCL6 CXCL6_HUMAN GCP 2 GCP-2 Granulocyte chemotactic protein 2 N-processed variant 3 SCYB6 Small inducible cytokine B6 Small inducible cytokine subfamily B (Cys X Cys) member 6 Small inducible cytokine subfamily B (Cys X Cys) member b Small-inducible cytokine B6 |
Images
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Fig1:
Sandwich ELISA analysis of human CXCL6 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723314) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CTGF protein (HA211111) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723315, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CXCL6 in human serum samples. The concentrations of CXCL6 were measured in duplicates, interpolated from the CXCL6 standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL6 concentration was determined to be 35.5 pg/ml in human serum. |
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Fig3:
Interpolated concentrations of spiked CXCL6 in human cell culture media samples. The concentrations of CXCL6 were measured in duplicates, interpolated from the CXCL6 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.