Human M-CSF Recombinant Rabbit Monoclonal Antibody [PSH11-37]
cat.: HA723324
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH11-37 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human M-CSF aa 33-255 (HA210918). |
| Positive control: | Recombinant Human M-CSF protein (HA210918). |
| Subcellular location: | Cell membrane. Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-38] to Human M-CSF antibody (Detector) (HA723325) and Recombinant Human M-CSF protein (HA210593) as the standard. The reference range value is 39.1-5,000 pg/mL. |
| Uniprot #: | SwissProt: P09603 Human |
| Alternative names: | Colony stimulating factor 1 (macrophage) Colony stimulating factor 1 Colony stimulating factor macrophage specific CSF 1 CSF-1 CSF1 CSF1_HUMAN Csfm Lanimostim M CSF M-CSF Macrophage Colony Stimulating Factor 1 Macrophage colony stimulating factor MCSF MGC31930 Processed macrophage colony-stimulating factor 1 |
Images
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Fig1:
Sandwich ELISA analysis of human M-CSF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723324) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human M-CSF protein (HA210918) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA72332, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native M-CSF in MG-63 cell culture supernatant and human urine samples. The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are MG-63 cell culture supernatant 20% and human urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 16,640 pg/ml in MG-63 cell culture supernatant and 738 pg/ml in human urine samples. |
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Fig3:
Interpolated concentrations of spiked M-CSF in human cell culture media samples. The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.