Biotin Conjugated Human/Mouse/Rat NF-L Recombinant Rabbit Monoclonal Antibody [PSH11-44]
cat.: HA723335B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH11-44 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NF-L protein aa 62-407 (HA211087). |
| Positive control: | Recombinant Human NF-L protein (HA211087). |
| Subcellular location: | Cell projection, axon, Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-43] to Human NF-L antibody (Capture) (HA723333) and Recombinant Human NF-L protein (HA211087) as the standard. The reference range value is 156-10,000pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P07196 Human | P08551 Mouse | P19527 Rat |
| Alternative names: | 68 kDa neurofilament protein 68kDa Neurofilament 68kDa neurofilament protein CMT1F CMT2E FLJ53642 Light molecular weight neurofilament protein NEFL Neurofilament light Neurofilament light polypeptide 68kDa Neurofilament light polypeptide Neurofilament protein, light chain Neurofilament subunit NF L Neurofilament triplet L protein NF-L NF68 NFL NFL_HUMAN |
Images
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Fig1:
Standard curve of human NF-L matched pair antibodies Sandwich ELISA analysis of human NF-L matched pair antibodiesElisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723333) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NF-L protein (HA211087) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723335B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native NF-L in mouse brain, rat brain and U-87 MG extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NF-L was measured in duplicate at different sample concentrations and interpolated from the NF-L standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NF-L concentration was determined to be 934,886 pg/mL in mouse brain and 406.883 pg/mL in rat brain tissue extract. There was no detectable signal in U-87 MG cell extract. |
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Fig3:
Interpolated concentrations of spiked NF-L in cell culture media samples. The concentrations of NF-L were measured in duplicates, interpolated from the NF-L standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.