FAM38A / PIEZO1 Recombinant Rabbit Monoclonal Antibody [PSH11-49]
cat.: HA723341
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH11-49 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 292 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse PIEZO1 aa 2,214-2,457. |
| Positive control: | 293T transfected with mPiezo1 cell lysate. |
| Subcellular location: | Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Cell membrane, Cell projection, lamellipodium membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:200 |
| Uniprot #: | SwissProt: E2JF22 Mouse |
| Alternative names: | Piezo-type mechanosensitive ion channel component 1 Protein FAM38A Piezo1 Fam38a |
Images
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Fig1:
Western blot analysis of FAM38A / PIEZO1 on different lysates with Rabbit anti-FAM38A / PIEZO1 antibody (HA723341) at 1/2,000 dilution. Lane 1: 293T transfected with mPiezo1 cell lysate Lane 2: 293T transfected with mPiezo2 cell lysate (negative) Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes 50 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723341) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of 293T cells labeling FAM38A / PIEZO1 with Rabbit anti-FAM38A / PIEZO1 antibody (HA723341) at 1/200 dilution. 293T cells, transfected with GFP-tagged mouse Piezo1 (top, positive) / tag-free mouse Piezo2 (bottom, negative) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM38A / PIEZO1 antibody (HA723341) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.