Bik Recombinant Rabbit Monoclonal Antibody [PSH11-51]
cat.: HA723343
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH11-51 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Bik aa 1-160. |
| Positive control: | Ramos cell lysate, Raji cell lysate, THP-1 cell lysate, Ramos. |
| Subcellular location: | Endomembrane system, Mitochondrion membrane. |
| Recommended Dilutions:
WB IF-Cell IP |
1:10,000 1:100 1-2μg/sample |
| Uniprot #: | SwissProt: Q13323 Human |
| Alternative names: | Apoptosis inducer NBK BBC1 Bcl-2-interacting killer BCL2 interacting killer bhikhari BIK Bik-like killer protein BIK_HUMAN BIP 1 BiP1 BP 4 BP4 cb 60 NBK |
Images
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Fig1:
Western blot analysis of Bik on different lysates with Rabbit anti-Bik antibody (HA723343) at 1/10,000 dilution. Lane 1: Ramos cell lysate Lane 2: SK-Br-3 cell lysate (negative) Lane 3: Raji cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723343) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of Ramos cells labeling Bik with Rabbit anti-Bik antibody (HA723343) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Bik antibody (HA723343) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Bik was immunoprecipitated from 0.2 mg Ramos cell lysate with HA723343 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723343 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Ramos cell lysate (input) Lane 2: HA723343 IP in Ramos cell lysate Lane 3: Rabbit IgG instead of HA723343 in Ramos cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 20 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.