Nectin 2 Recombinant Rabbit Monoclonal Antibody [PSH11-54]
cat.: HA723346
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH11-54 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Nectin 2 aa 1-360. |
| Positive control: | MCF7 cell lysate, HT-29 cell lysate, MCF7. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q92692 Human |
| Alternative names: | CD 112 CD112 CD112 antigen Herpes virus entry mediator B Herpes virus entry protein B Herpesvirus entry mediator B Herpesvirus entry protein B Hve B HveB Nectin-2 Nectin2 Poliovirus receptor like 2 Poliovirus receptor related 2 (herpesvirus entry mediator B) Poliovirus receptor related 2 Poliovirus receptor related protein 2 Poliovirus receptor-related protein 2 PRR 2 PRR2 PVRL 2 PVRL2 PVRL2_HUMAN PVRR 2 PVRR2 |
Images
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Fig1:
Western blot analysis of Nectin 2 on different lysates with Rabbit anti-Nectin 2 antibody (HA723346) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MOLT-4 cell lysate (negative) Lane 3: HT-29 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 58-75 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723346) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of MCF7 (positive) and MOLT-4 (negative) labeling Nectin 2 with Rabbit anti-Nectin 2 antibody (HA723346) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Nectin 2 antibody (HA723346) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of MOLT-4 (left, negative) and MCF7 (right, positive) cells labeling Nectin 2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723346, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.