Human IL3RA/CD123 Recombinant Rabbit Monoclonal Antibody [PSH11-57]
cat.: HA723351
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH11-57 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL3RA/CD123 aa 19-305 (HA211048). |
| Positive control: | Recombinant Human IL3RA/CD123 protein (HA211048). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-58] to Human IL3RA/CD123 antibody (Detector) (HA723352) and Recombinant Human IL3RA/CD123 protein (HA211048) as the standard. The reference range value is 0.625-20 ng/mL. |
| Uniprot #: | SwissProt: P26951 Human |
| Alternative names: | CD123 CD123 antigen hIL 3Ra hIL-3Ra hIL3Ra IL 3 receptor alpha SP2 isoform IL 3R alpha IL-3 receptor subunit alpha IL-3R subunit alpha IL-3R-alpha IL-3RA IL3 Receptor alpha IL3R Il3ra IL3RA_HUMAN IL3RAX IL3RAY IL3RX IL3RY Interleukin 3 receptor alpha chain Interleukin 3 receptor, alpha (low affinity) Interleukin 3 receptor, alpha Interleukin-3 Receptor alpha Interleukin-3 receptor subunit alpha Interleukin3 receptor Interleukin3 receptor, Y-chromosomal |
Images
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Fig1:
Sandwich ELISA analysis of human CD123 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723351) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL3RA/CD123 protein (HA211048) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723352, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.