Biotin Conjugated Human FOLR1 Recombinant Rabbit Monoclonal Antibody [PSH11-60]
cat.: HA723356B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH11-60 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FOLR1 aa 25-233. |
| Positive control: | Recombinant Human FOLR1 protein (HA211055). |
| Subcellular location: | Cell membrane. Cytoplasmic vesicle. Endosome. Membrane. Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-59] to Human FOLR1 antibody (Capture) (HA723354) and Recombinant Human FOLR1 protein (HA211055) as the standard. The reference range value is 0.625-10 mg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P15328 Human |
| Alternative names: | adult Adult folate binding protein Adult folate-binding protein FBP Folate Binding Protein Folate Receptor 1 Adult Folate receptor 1 Folate Receptor 1 Precursor Folate receptor adult Folate receptor alpha Folate receptor FOLR FOLR1 FOLR1_HUMAN FR alpha FR-alpha FRalpha KB cells FBP MOV18 Ovarian cancer associated antigen Ovarian tumor associated antigen Ovarian tumor associated antigen MOv18 Ovarian tumor-associated antigen MOv18 |
Images
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Fig1:
Sandwich ELISA analysis of human FOLR1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723354) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant human FOLR1 protein (HA211055) starting from 3,000 pg/ml to 0 pg/ml and detect antibody (HA723356B, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.