HA723357

IFT88 Recombinant Rabbit Monoclonal Antibody [PSH11-61]

cat.: HA723357

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Monkey
Applications:	WB, IHC-P, IF-Cell
Clonality:	Monoclonal
Clone number:	PSH11-61

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 93 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human IFT88 aa 575-824.
Positive control:	HEK-293 cell lysate, Jurkat cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, A549 cell lysate, HepG2 cell lysate, COS-1 cell lysate, human testis tissue, rat testis tissue, NIH/3T3, C6.
Subcellular location:	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, Cell projection, cilium, cilium basal body, flagellum.
Recommended Dilutions: WB IHC-P IF-Cell	1:2,000-1:5,000 1:1,000 1:50
Uniprot #:	SwissProt: Q13099 Human \| Q61371 Mouse Entrez Gene: 305918 Rat
Alternative names:	D13S1056E DAF19 hTg737 Ift88 IFT88_HUMAN Intraflagellar transport 88 homolog Intraflagellar transport protein 88 homolog MGC26259 Polaris homolog Probe hTg737 (polycystic kidney disease, autosomal recessive) Recessive polycystic kidney disease protein Tg737 homolog RP11-172H24.2 Tetratricopeptide repeat domain 10 Tetratricopeptide repeat protein 10 TG737 TPR repeat protein 10 TTC10

Images

Fig1: Western blot analysis of IFT88 on different lysates with Rabbit anti-IFT88 antibody (HA723357) at 1/5,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HeLa cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: C6 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 93 kDa
Observed band size: 93 kDa

Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723357) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of IFT88 on different lysates with Rabbit anti-IFT88 antibody (HA723357) at 1/2,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: C6 cell lysate
Lane 6: COS-1 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 93 kDa
Observed band size: 93 kDa

Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723357) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-IFT88 antibody (HA723357) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723357) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-IFT88 antibody (HA723357) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723357) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunocytochemistry analysis of NIH/3T3 cells labeling IFT88 with Rabbit anti-IFT88 antibody (HA723357) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFT88 antibody (HA723357) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Fig6: Immunocytochemistry analysis of C6 cells labeling IFT88 with Rabbit anti-IFT88 antibody (HA723357) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFT88 antibody (HA723357) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.