TIMP2 Recombinant Rabbit Monoclonal Antibody [PSH11-62]
cat.: HA723358
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH11-62 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TIMP2 aa 1-220. |
| Positive control: | HeLa cell lysate, SW480 cell lysate, COS-1 cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P16035 Human |
| Alternative names: | CSC 21K CSC-21K CSC21K Metalloproteinase inhibitor 2 Metalloproteinase inhibitor 2 precursor TIMP 2 TIMP metallopeptidase inhibitor 2 TIMP-2 TIMP2 TIMP2_HUMAN Tissue Inhibitor of Metalloproteinase 2 Tissue inhibitor of metalloproteinases 2 |
Images
|
Fig1:
Western blot analysis of TIMP2 on different lysates with Rabbit anti-TIMP2 antibody (HA723358) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 21 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723358) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of TIMP2 on different lysates with Rabbit anti-TIMP2 antibody (HA723358) at 1/2,000 dilution. Lane 1: SW480 cell lysate Lane 2: HeLa cell lysate Lane 3: COS-1 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 24 kDa Observed band size: 21 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723358) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.