BRN3A Recombinant Rabbit Monoclonal Antibody [PSH11-65]
cat.: HA723361
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB, mIHC |
| Clonality: | Monoclonal |
| Clone number: | PSH11-65 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse BRN3A aa 1-421. |
| Positive control: | Mouse embryonic brain tissue, mouse embryonic cochlear vestibular ganglion tissue, E14.5 mouse embryonic eye tissue, E14.5 rat embryo tissue, E14.0 mouse embryonic brain tissue lysates. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
IHC-Fr IHC-P WB mIHC |
1:500 1:200-1:1,000 1:1,000 1:200-1:500 |
| Uniprot #: | SwissProt: P17208 Mouse | P20266 Rat |
| Alternative names: | Brain specific homeobox/POU domain protein 3A Brain-3A Brain-specific homeobox/POU domain protein 3A Brn 3.0 BRN 3A Brn-3A Brn3 BRN3A class 4 FLJ13449 Homeobox/POU domain protein RDC 1 Homeobox/POU domain protein RDC-1 Homeobox/POU domain protein RDC1 Oct T1 Oct-T1 OctT1 PO4F1_HUMAN POU class 4 homeobox 1 POU domain POU Domain Class 4 Transcription Factor 1 Pou4f1 RDC 1 RDC1 transcription factor 1 |
Images
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Fig1:
mIHC analysis of mouse retina tissue (Frozen section) with BRN3A (HA723361, 1/500) and βIII tubulin (HA723738, 1/5,000). The immunostaining was performed with the IRISKitCmTSA Kit (900802). Blocking with 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
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Fig2:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Retina Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA723361, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig3:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: E14.5 embryonic brain Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA723361, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig4:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: E14.5 embryonic cochlear vestibular ganglion Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA723361, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Retina Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA723361, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Retina Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA723361, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig7:
Western blot analysis of BRN3A on E14.0 mouse embryonic brain tissue lysates with Rabbit anti-BRN3A antibody (HA723361) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 47 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723361) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.