PGAM5 Recombinant Rabbit Monoclonal Antibody [PSH11-68]
cat.: HA723364
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH11-68 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PGAM5 aa 30-289. |
| Positive control: | HeLa cell lysate, A549cell lysate, HepG2 cell lysate, PANC-1 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate. |
| Subcellular location: | Mitochondrion outer membrane, Mitochondrion inner membrane. |
| Recommended Dilutions:
WB |
1:10,000 |
| Uniprot #: | SwissProt: Q96HS1 Human | Q8BX10 Mouse | Q562B5 Rat |
| Alternative names: | Bcl-XL-binding protein v68 BXLBv68 MGC5352 mitochondrial PGAM5 PGAM5_HUMAN Phosphoglycerate mutase family member 5 Serine/threonine protein phosphatase PGAM5 mitochondrial Serine/threonine-protein phosphatase PGAM5 |
Images
|
Fig1:
Western blot analysis of PGAM5 on different lysates with Rabbit anti-PGAM5 antibody (HA723364) at 1/10,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549cell lysate Lane 3: HepG2 cell lysate Lane 4: PANC-1 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: MEF cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723364) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.