HRP Conjugated Human CTGF Recombinant Rabbit Monoclonal Antibody [PSH11-16]
cat.: HA723375H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH11-16 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CTGF aa 27-349. |
| Positive control: | Recombinant Human CTGF protein (HA211051). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-15] to Human CTGF antibody (Capture) (HA723296) and Recombinant Human CTGF protein (HA211051) as the standard. |
| Uniprot #: | SwissProt: P29279 Human |
| Alternative names: | CCN 2 CCN family member 2 CCN2 Connective tissue growth factor Ctgf CTGF_HUMAN Hcs 24 Hcs24 Hypertrophic chondrocyte specific protein 24 Hypertrophic chondrocyte-specific gene product 24 Hypertrophic chondrocyte-specific protein 24 IBP-8 IGF-binding protein 8 IGFBP-8 IGFBP8 Insulin like Growth Factor Binding Protein 8 Insulin-like growth factor-binding protein 8 MGC102839 NOV 2 NOV2 |
Images
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Fig1:
Sandwich ELISA analysis of human CTGF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723296) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CTGF protein (HA211051) starting from 333.33 ng/ml to 0 pg/ml and detect antibody (HA723375H, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.