Human CCL3 / MIP-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH11-78]
cat.: HA723380
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH11-78 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 10 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL3 aa 24-92 (HA210901). |
| Positive control: | Recombinant Human CCL3 / MIP-1 alpha protein HA210901 (HA210901). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-77] to Human CCL3 / MIP-1 alpha antibody (Detector) (HA723378) and Recombinant Human CCL3 / MIP-1 alpha protein (HA210901) as the standard. The reference range value is 3.9-1000 pg/ml. |
| Uniprot #: | SwissProt: P10147 Human |
| Alternative names: | C C motif chemokine 3 CCL 3 CCL3 CCL3_HUMAN Chemokine (C C motif) ligand 3 Chemokine C C motif ligand 3 Chemokine ligand 3 G0/G1 switch regulatory protein 19 1 G0/G1 switch regulatory protein 19-1 G0S19 1 G0S19 1 protein Heparin binding chemotaxis protein L2G25B LD78 alpha LD78-alpha(4-69) LD78alpha Macrophage inflammatory protein 1 alpha Macrophage inflammatory protein 1-alpha macrophage inflammatory protein 1a MIP 1 alpha MIP 1A MIP-1-alpha MIP-1-alpha(4-69) MIP1 alpha MIP1A PAT 464.1 SCYA 3 SCYA3 SIS alpha SIS beta SIS-beta Small inducible cytokine A3 small inducible cytokine A3 (homologous to mouse Mip-1a) Small-inducible cytokine A3 Tonsillar lymphocyte LD78 alpha protein |
Images
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Fig1:
Sandwich ELISA analysis of human CCL3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723380) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL3 / MIP-1 alpha protein (HA210901) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA723378, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CLL3 in human THP-1 supernatant treated or untreated with PMA and LPS. Interpolated concentration of native CLL3 was measured in duplicate at different sample concentrations and interpolated from the CLL3 standard curves. Undiluted samples are as follows: untreated 100% and treated 12.5%. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CLL3 concentration was determined to be 25 pg/ml in untreated THP-1 cell culture supernatant and 5,277 pg/mL in treated THP-1 supernatant. |
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Fig3:
Interpolated concentrations of spiked CLL3 in cell culture media samples. The concentrations of CLL3 were measured in duplicates, interpolated from the CLL3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.