Human CNTF Recombinant Rabbit Monoclonal Antibody [PSH11-79]
cat.: HA723381
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH11-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CNTF aa 1-200. |
| Positive control: | Recombinant Human CNTF protein (HA210944). |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-80] to Human CNTF antibody (Detector) (HA723382) and Recombinant Human CNTF protein (HA210944) as the standard. The reference range value is 15.6-2,000 pg/ml. |
| Uniprot #: | SwissProt: P26441 Human |
| Alternative names: | Ciliary neurotrophic factor CNTF CNTF_HUMAN HCNTF |
Images
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Fig1:
Sandwich ELISA analysis of human CNTF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723381) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CNTF protein (HA210944) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723382, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CNTF in rat hypothalamus extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CNTF was measured in duplicate at different sample concentrations and interpolated from the CNTF standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CNTF concentration was determined to be 182 pg/mL in rat hypothalamus tissue extract. |
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Fig3:
Interpolated concentrations of spiked CNTF in cell culture media samples. The concentrations of CNTF were measured in duplicates, interpolated from the CNTF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.