Human Siglec 7 Recombinant Rabbit Monoclonal Antibody [PSH11-88]
cat.: HA723395
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH11-88 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Siglec 7 aa 19-353. |
| Positive control: | Recombinant Human Siglec 7 protein (HA210942). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-87] to Human Siglec 7 antibody (Capture) (HA723393) and Recombinant Human Siglec 7 protein (HA210942) as the standard. The reference range value is 62.5-8,000 pg/ml. |
| Uniprot #: | SwissProt: Q9Y286 Human |
| Alternative names: | Adhesion inhibitory receptor molecule 1 Adhesion inhibitory receptor molecule 1, siglec 7 AIRM 1 AIRM-1 AIRM1 CD328 CD328 antigen CDw328 D siglec D-siglec HLDA 8 p75 p75/AIRM1 QA79 QA79 membrane protein Sialic acid binding Ig like lectin 7 Sialic acid binding immunoglobulin like lectin 7 Sialic acid-binding Ig-like lectin 7 SIGL7_HUMAN Siglec-7 SIGLEC19P SIGLEC7 SIGLECP2 |
Images
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Fig1:
Sandwich ELISA analysis of human Siglec 7 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723393) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Siglec 7 protein (HA210942) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723395, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Siglec-7 in U937 and Jurkat extract samples based on a 1,000 µg/ml extract load. The concentrations of Siglec-7 were measured in duplicates, interpolated from the Siglec-7 standard curve and corrected for sample dilution. Undiluted samples are U937 extract 25% and Jurkat extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Siglec-7 concentration was determined to be 1,872 pg/ml in U937 extract and undetectable in Jurkat extract. |
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Fig3:
Interpolated concentrations of spiked Siglec-7 in human cell culture media samples. The concentrations of Siglec-7 were measured in duplicates, interpolated from the Siglec-7 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.