Twist1 Recombinant Rabbit Monoclonal Antibody [PSH11-98]
cat.: HA723411
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH11-98 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Twist1 aa 1-50. |
| Positive control: | SH-SY5Y cell lysate, A375 cell lysate, PC-3M cell lysate, MCF7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, E14.0 mouse embryonic brain tissue lysate, Rat embryo tissue lysate, LLC cell lysate, Mouse embryo tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q15672 Human | P26687 Mouse Entrez Gene: 85489 Rat |
| Alternative names: | ACS3 B-HLH DNA binding protein bHLHa38 BPES2 BPES3 Class A basic helix-loop-helix protein 38 CRS CRS1 CSO H-twist OTTHUMP00000116043 SCS Twist basic helix loop helix transcription factor 1 Twist family bHLH transcription factor 1 Twist homolog 1 (Drosophila) Twist homolog 1 TWIST homolog of drosophila Twist related protein 1 Twist-related protein 1 TWIST1 TWST1_HUMAN |
Images
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Fig1:
Western blot analysis of Twist1 on different lysates with Rabbit anti-Twist1 antibody (HA723411) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: A375 cell lysate Lane 3: PC-3M cell lysate Lane 4: MCF7 cell lysate Lane 5: HeLa cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: E14.0 mouse embryonic brain tissue lysate Lane 8: Rat embryo tissue lysate Lane 9: LLC cell lysate Lane 10: Mouse embryo tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 24 kDa Exposure time: Lane 1-8: 1 minute; Lane 9-10: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.