Stanniocalcin 2 / STC-2 Recombinant Rabbit Monoclonal Antibody [PSH12-01]
cat.: HA723414
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH12-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human STC-2 aa 1-302. |
| Positive control: | MCF7 cell lysate, T-47D cell lysate, HepG2 cell lysate, HeLa treated with 10μM BFA for 24 hours cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: O76061 Human |
| Alternative names: | Stanniocalcin 2 Stanniocalcin related protein Stanniocalcin-2 Stanniocalcin-related protein Stanniocalcin2 STC 2 STC related protein STC-2 STC-related protein STC2 STC2_HUMAN STCRP |
Images
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Fig1:
Western blot analysis of Stanniocalcin 2 / STC-2 on different lysates with Rabbit anti-Stanniocalcin 2 / STC-2 antibody (HA723414) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: T-47D cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 36/40 kDa Exposure time: Lane 1-2: 25 seconds; Lane 3: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723414) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Stanniocalcin 2 / STC-2 on different lysates with Rabbit anti-Stanniocalcin 2 / STC-2 antibody (HA723414) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10μM BFA for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 36/40 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723414) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Stanniocalcin 2 / STC-2 was immunoprecipitated from 0.2 mg T-47D cell lysate with HA723414 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723414 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: T-47D cell lysate (input) Lane 2: HA723414 IP in T-47D cell lysate Lane 3: Rabbit IgG instead of HA723414 in T-47D cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 minutes; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.