Biotin Conjugated Human Leptin Recombinant Rabbit Monoclonal Antibody [PSH12-07]
cat.: HA723423B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH12-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Leptin aa 22-167. |
| Positive control: | Recombinant Human Leptin protein (HA210961). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-06] to Human Leptin antibody (Capture) (HA72342) and Recombinant Human Leptin protein (HA210961) as the standard. The reference range value is 15.6-2,000 pg/ml. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P41159 Human |
| Alternative names: | FLJ94114 LEP LEP_HUMAN LEPD Leptin (murine obesity homolog) Leptin (obesity homolog, mouse) Leptin Leptin Murine Obesity Homolog Leptin Precursor Obesity Factor OB Obese protein Obese, mouse, homolog of Obesity Obesity factor Obesity homolog mouse Obesity Murine Homolog Leptin OBS OTTHUMP00000212285 |
Images
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Fig1:
Sandwich ELISA analysis of human Leptin matched pair antibodies Capture: HA723421, Human Leptin Rabbit mAb [PSH12-06] Detector: HA723422, Human Leptin Rabbit mAb [PSH12-07] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723421) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Leptin protein (HA210961) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723421, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Leptin in human serum samples. Capture: HA723421, Human Leptin Rabbit mAb [PSH12-06] Detector: HA723422, Human Leptin Rabbit mAb [PSH12-07] The concentrations of Leptin were measured in duplicate, interpolated from the Leptin standard curves and corrected for sample dilution. Undiluted samples are human serum 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Leptin concentration was determined to be 9,115 pg/ml in human serum. |
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Fig3:
Interpolated concentrations of spiked Leptin in human cell culture media samples. Capture: HA723421, Human Leptin Rabbit mAb [PSH12-06] Detector: HA723422, Human Leptin Rabbit mAb [PSH12-07] The concentrations of Leptin were measured in duplicates, interpolated from the Leptin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.