ENPP1 / PC1 Recombinant Rabbit Monoclonal Antibody [PSH12-11]
cat.: HA723427
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC |
| Clonality: | Monoclonal |
| Clone number: | PSH12-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 105 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ENPP1 aa 576-925. |
| Positive control: | HepG2 cell lysate, MDA-MB-231 cell lysate, Huh7 cell lysate, human liver tissue. |
| Subcellular location: | Cell membrane, Basolateral cell membrane, Secreted. |
| Recommended Dilutions:
WB IHC |
1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P22413 Human |
| Alternative names: | Alkaline phosphodiesterase 1 ARHR2 COLED E-NPP 1 Ectonucleotide pyrophosphatase/phosphodiesterase 1 Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ENPP1 ENPP1_HUMAN Ly 41 antigen M6S1 Membrane component chromosome 6 surface marker 1 NPP1 NPPase NPPS Nucleotide pyrophosphatase PC 1 PC-1 PCA1 PDNP1 Phosphodiesterase I/nucleotide pyrophosphatase 1 Plasma cell membrane glycoprotein 1 Plasma-cell membrane glycoprotein PC-1 |
Images
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Fig1:
Western blot analysis of ENPP1 / PC1 on different lysates with Rabbit anti-ENPP1 / PC1 antibody (HA723427) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: Huh7 cell lysate Lane 4: THP-1 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 105 kDa Observed band size: 140 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723427) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ENPP1 / PC1 antibody (HA723427) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723427) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.