TNF Receptor II Recombinant Rabbit Monoclonal Antibody [PSH12-12]
cat.: HA723428
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | FC |
| Clonality: | Monoclonal |
| Clone number: | PSH12-12 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TNF Receptor II aa 1-257. |
| Positive control: | K-562. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
FC |
1:500 |
| Uniprot #: | SwissProt: P20333 Human |
| Alternative names: | CD120b p75 p75 TNF receptor p75TNFR p80 TNF alpha receptor p80 TNF-alpha receptor Soluble TNFR1B variant 1 TBP-2 TBPII TNF R II TNF R2 TNF R75 TNF-R2 TNF-RII TNFBR TNFR-II TNFR1B TNFR2 TNFR80 TNFRII Tnfrsf1b TNR1B_HUMAN Tumor necrosis factor beta receptor Tumor necrosis factor binding protein 2 Tumor necrosis factor receptor 2 Tumor necrosis factor receptor superfamily member 1B Tumor necrosis factor receptor type II Tumor necrosis factor-binding protein 2 |
Images
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Fig1:
Flow cytometric analysis of 293T (left, low expression) and K-562 (right, positive) cells labeling TNF Receptor II. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723428, 1/500) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.