HRP Conjugated Human Von Willebrand Factor Recombinant Rabbit Monoclonal Antibody [PSH09-04]
cat.: HA723431H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH09-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human von Willebrand factor protein aa 764-1263 (HA210992). |
| Positive control: | Recombinant human von Willebrand factor protein (HA210992). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-03] to Human Von Willebrand Factor antibody (Capture) (HA723058) and Recombinant Human Von Willebrand Factor protein (HA210992) as the standard. |
| Uniprot #: | SwissProt: P04275 Human |
| Alternative names: | Coagulation factor VIII Coagulation factor VIII VWF F8VWF Factor VIII related antigen von Willebrand antigen 2 von Willebrand antigen II Von Willebrand disease Von Willebrand factor precursor VWD vWF VWF_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of human Von Willebrand Factor matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723058) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human Von Willebrand Factor protein (HA210992) starting from 1,000 ng/ml to 0 ng/ml and detect antibody (HA723431H, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.