Human IGFBP3 Recombinant Rabbit Monoclonal Antibody [PSH12-17]
cat.: HA723433
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-17 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IGFBP3 aa 28-291. |
| Positive control: | Recombinant Human IGFBP3 protein (HA210585). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-16] to Human IGFBP3 antibody (Capture) (HA723432) and Recombinant Human IGFBP3 protein (HA210585) as the standard. The reference range value is 31.5-4,000 pg/ml. |
| Uniprot #: | SwissProt: P17936 Human |
| Alternative names: | Acid stable subunit of the 140 K IGF complex Binding protein 29 Binding protein 53 BP 53 BP53 Growth hormone dependent binding protein IBP 3 IBP-3 IBP3 IBP3_HUMAN IGF binding protein 3 IGF-binding protein 3 IGFBP 3 IGFBP-3 IGFBP3 Insulin Like Growth Factor Binding Protein 3 Insulin-like growth factor binding protein 3 precursor Insulin-like growth factor-binding protein 3 |
Images
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Fig1:
Sandwich ELISA analysis of human IGFBP3 matched pair antibodies Capture: HA723432, Human Leptin Rabbit mAb [PSH12-16] Detector: HA723433, Human Leptin Rabbit mAb [PSH12-17] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723432) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IGFBP3 protein (HA210585) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723433, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IGFBP-3 in A431, A549 and HT-29 cell culture supernatant. Capture: HA723432, Human Leptin Rabbit mAb [PSH12-16] Detector: HA723433, Human Leptin Rabbit mAb [PSH12-17] The concentrations of IGFBP-3 were measured in duplicates, interpolated from the IGFBP-3 standard curve and corrected for sample dilution. Undiluted samples are A431 cell culture supernatant 2%, A549 cell culture supernatant 25% and HT-29 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP-3 concentration was determined to be 131.3 ng/ml in A431 cell culture supernatant, 8.04 ng/ml in A549 cell culture supernatant and undetectable in HT-29 cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked IGFBP-3 in human cell culture media samples. Capture: HA723432, Human Leptin Rabbit mAb [PSH12-16] Detector: HA723433, Human Leptin Rabbit mAb [PSH12-17] The concentrations of IGFBP-3 were measured in duplicates, interpolated from the IGFBP-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.