HA723435

Human PAI1 Recombinant Rabbit Monoclonal Antibody [PSH12-18]

cat.: HA723435

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	ELISA(Cap), ELISA(Det)
Clonality:	Monoclonal
Clone number:	PSH12-18

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4).
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Isotype:	IgG
Immunogen:	Recombinant protein within Human PAI1 aa 24-402 (HA210982).
Positive control:	Recombinant Human PAI1 protein (HA210982).
Subcellular location:	Secreted.
Recommended Dilutions: ELISA(Cap) ELISA(Det)	Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-19] to Human PAI1 antibody (Detector) (HA723436) and Recombinant Human PAI1 protein (HA210982) as the standard. The reference range value is 78.1-10,000 pg/mL. Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-20] to Human PAI1 antibody (Capture) (HA723438) and Recombinant Human PAI1 protein (HA210982) as the standard. The reference range value is 78.1-10,000 pg/mL.
Uniprot #:	SwissProt: P05121 Human
Alternative names:	Clade E Endothelial plasminogen activator inhibitor Nexin Nexin plasminogen activator inhibitor type 1 PAI 1 PAI PAI-1 PAI1_HUMAN PLANH1 Plasminogen activator inhibitor 1 Plasminogen activator inhibitor type 1 Serine (or cysteine) proteinase inhibitor Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 Serine proteinase inhibitor clade E member 1 serpin Serpin E1 Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 Serpin peptidase inhibitor clade E Serpine 1 SERPINE1

Images

	Fig1: Sandwich ELISA analysis of Human PAI1 matched pair antibodies Capture: HA723435, Human PAI1 Rabbit mAb [PSH12-18] Detector: HA723436, Human PAI1 Rabbit mAb [PSH12-19] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723435) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PAI1 protein (HA210982) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723436, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
	Fig2: Interpolated concentrations of native PAI1 in human cell culture supernatant samples. Capture: HA723435, Human PAI1 Rabbit mAb [PSH12-18] Detector: HA723436, Human PAI1 Rabbit mAb [PSH12-19] Interpolated concentration of native PAI1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 1276000pg/mL in HUVEC sups, 158000 pg/ml in HepG2 cell culture supernatant.
	Fig3: Interpolated concentrations of spiked PAI1 in cell culture media samples. Capture: HA723435, Human PAI1 Rabbit mAb [PSH12-18] Detector: HA723436, Human PAI1 Rabbit mAb [PSH12-19] The concentrations of PAI1 were measured in duplicates, interpolated from the PAI1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
	Fig4: Sandwich ELISA analysis of Human PAI1 matched pair antibodies Capture: HA723438, Human PAI1 Rabbit mAb [PSH12-20] Detector: HA723435, Human PAI1 Rabbit mAb [PSH12-18] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723438) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PAI1 protein (HA210982) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723435, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Fig5: Interpolated concentrations of native PAI1 in human cell culture supernatant samples.

Capture: HA723438, Human PAI1 Rabbit mAb [PSH12-20]
Detector: HA723435, Human PAI1 Rabbit mAb [PSH12-18]

Interpolated concentration of native PAI1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 371000 pg/mL in HUVEC sups, 51000 pg/ml in HepG2 cell culture supernatant.

Fig6: Interpolated concentrations of spiked PAI1 in cell culture media samples.

Capture: HA723438, Human PAI1 Rabbit mAb [PSH12-20]
Detector: HA723435, Human PAI1 Rabbit mAb [PSH12-18]

The concentrations of PAI1 were measured in duplicates, interpolated from the PAI1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.