HRP Conjugated Human PAI1 Recombinant Rabbit Monoclonal Antibody [PSH12-18]
cat.: HA723439H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH12-18 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PAI1 aa 24-402 (HA210982). |
| Positive control: | Recombinant Human PAI1 protein (HA210982). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-20] to Human PAI1 antibody (Capture) (HA723438) and Recombinant Human PAI1 protein (HA210982) as the standard. The reference range value is 234.3-15,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P05121 Human |
| Alternative names: | Clade E Endothelial plasminogen activator inhibitor Nexin Nexin plasminogen activator inhibitor type 1 PAI 1 PAI PAI-1 PAI1_HUMAN PLANH1 Plasminogen activator inhibitor 1 Plasminogen activator inhibitor type 1 Serine (or cysteine) proteinase inhibitor Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 Serine proteinase inhibitor clade E member 1 serpin Serpin E1 Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 Serpin peptidase inhibitor clade E Serpine 1 SERPINE1 |
Images
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Fig1:
Sandwich ELISA analysis of Human PAI1 matched pair antibodies Capture: HA723438, Human PAI1 Rabbit mAb [PSH12-20] Detector: HA723435, Human PAI1 Rabbit mAb [PSH12-18] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723438) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PAI1 protein (HA210982) starting from 15,000 pg/ml to 0 pg/ml and detect antibody (HA723435, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.