Mouse Factor XII Recombinant Rabbit Monoclonal Antibody [PSH12-21]
cat.: HA723440
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Factor XII aa 20-597 (HA210984). |
| Positive control: | Recombinant Mouse Factor XII protein (HA210984). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-22] to Mouse Factor XII antibody (Detector) (HA723441) and Recombinant Mouse Factor XII protein (HA210984) as the standard. The reference range value is 234-30,000 pg/mL. |
| Uniprot #: | SwissProt: Q80YC5 Mouse |
| Alternative names: | Factor XII Beta factor XIIa part 1 Beta factor XIIa part 2 Coagulation factor XII Coagulation factor XIIa heavy chain Coagulation factor XIIa light chain F12 F12 deficiency FA12_HUMAN Factor XII deficiency HAE3 HAEX HAF HAF deficiency Hageman factor |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723440) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (HA210984) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA723441, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Factor XII in mouse samples. Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 26,213,785 pg/mL in mouse serum and 24,389 pg/ml in mouse liver Supernatant. |
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Fig3:
Interpolated concentrations of spiked Factor XII in cell culture media samples. Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.