Biotin Conjugated Mouse CD68 Recombinant Rabbit Monoclonal Antibody [PSH12-27]
cat.: HA723451B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH12-27 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse CD68 aa 21-291 (HA211131). |
| Positive control: | Recombinant Mouse CD68 protein (HA211131). |
| Subcellular location: | Endosome membrane, Lysosome membrane; Cell membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-26] to Mouse CD68 antibody (Capture) (HA723449) and Recombinant Mouse CD68 protein (HA211131) as the standard. The reference range value is 31.3-4,000 pg/ml. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P31996 Mouse |
| Alternative names: | CD 68 CD68 CD68 antigen CD68 molecule CD68_HUMAN DKFZp686M18236 gp11 Gp110 LAMP4 Macrophage antigen CD68 (microsialin) MACROPHAGE ANTIGEN CD68 Macrosialin SCARD1 Scavenger receptor class D member 1 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse CD68 matched pair antibodies Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26] Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723449) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD68 protein (HA211131) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723450, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CD68 in mouse spleen, Raw264.7 and Neuro-2a extract samples based on a 1000 µg/ml extract load. Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26] Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27] Interpolated concentration of native CD68 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD68 concentration was determined to be 35353 pg/mL in mouse spleen extract,47421 pg/ml in Raw264.7 extract and undetectable in Neuro-2a extract. |
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Fig3:
Interpolated concentrations of spiked CD68 in cell culture media samples. Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26] Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27] The concentrations of CD68 were measured in duplicates, interpolated from the CD68 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.