Human Oxytocin-neurophysin 1 Recombinant Rabbit Monoclonal Antibody [PSH12-28]
cat.: HA723452
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-28 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Oxytocin-neurophysin 1 aa 20-125. |
| Positive control: | Recombinant Human Oxytocin-neurophysin 1 protein. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-29] to Human Oxytocin-neurophysin 1 antibody (Detector) (HA723453) and Recombinant Human Oxytocin-neurophysin 1 protein as the standard. The reference range value is 78.1-10,000 pg/mL. |
| Uniprot #: | SwissProt: P01178 Human |
| Alternative names: | MGC126890 MGC126892 Neurophysin 1 Neurophysin I Ocytocin OT OT NPI OXT Oxytocin Oxytocin Neurophysin preproprotein Oxytocin prepro (neurophysin I) Oxytocin prepropeptide Oxytocin-neurophysin 1 |
Images
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Fig1:
Sandwich ELISA analysis of human Oxytocin-neurophysin 1 matched pair antibodies Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28] Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723452) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Oxytocin-neurophysin 1 protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723453, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Oxytocin-neurophysin 1 in human serum samples. Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28] Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29] Interpolated concentration of native Oxytocin-neurophysin 1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Oxytocin-neurophysin 1 concentration was determined to be 1368 pg/mL in Human serum. |
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Fig3:
Interpolated concentrations of spiked Oxytocin-neurophysin 1 in cell culture media samples. Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28] Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29] The concentrations of Oxytocin-neurophysin 1 were measured in duplicates, interpolated from the Oxytocin-neurophysin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.