KCNA5 / KV1-5 Recombinant Rabbit Monoclonal Antibody [PSH12-30]
cat.: HA723455
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH12-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rat KCNA5 aa 249-298 / 602. |
| Positive control: | PANC-1 cell lysate, Mouse hippocampus tissue lysate, Mouse heart tissue lysate, Rat hippocampus tissue lysate, Rat heart tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P22460 Human | Q61762 Mouse | P19024 Rat |
| Alternative names: | ATFB7 cardiac potassium channel HCK1 HK2 HPCN1 insulinoma and islet potassium channel KCNA5 KCNA5_HUMAN KV1.5 PCN1 potassium channel 1 Potassium channel, insulinoma and islet cell Potassium channel, voltage-gated, shaker-related subfamily, member 5 Potassium voltage gated channel subfamily A member 5 Potassium voltage-gated channel subfamily A member 5 Voltage-gated potassium channel HK2 voltage-gated potassium channel protein Kv1.5 Voltage-gated potassium channel subunit Kv1.5 |
Images
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Fig1:
Western blot analysis of KCNA5 / KV1-5 on different lysates with Rabbit anti-KCNA5 / KV1-5 antibody (HA723455) at 1/1,000 dilution. Lane 1: PANC-1 cell lysate(no heat) (15 µg/Lane) Lane 2: Mouse hippocampus tissue lysate (no heat) (20 µg/Lane) Lane 3: Mouse heart tissue lysate (no heat) (20 µg/Lane) Lane 4: Mouse liver tissue lysate (negative) (20 µg/Lane) Lane 5: Rat hippocampus tissue lysate (20 µg/Lane) Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane) Lane 7: Rat heart tissue lysate (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 1 minute 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723455) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.