Human Poliovirus Receptor/PVR Recombinant Rabbit Monoclonal Antibody [PSH12-54]
cat.: HA723464
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-54 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD155 / PVR aa 21-343 (HA210957). |
| Positive control: | Recombinant Human Poliovirus Receptor/PVR protein (HA210957). |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-55] to Human Poliovirus Receptor/PVRn antibody (Detector) (HA723465) and Recombinant Human Poliovirus Receptor/PVR protein (HA210957) as the standard. The reference range value is 62.5-8,000 pg/mL. |
| Uniprot #: | SwissProt: P15151 Human |
| Alternative names: | CD155 CD155 antigen FLJ25946 HVED mE4 NECL 5 Necl-5 Necl5 Nectin like 5 Nectin like protein 5 Nectin-like protein 5 Ortholog of mouse Tage4 Poliovirus receptor PVR PVR_HUMAN PVS Taa1 Tage 4 TAGE4 |
Images
|
Fig1:
Sandwich ELISA analysis of Human Poliovirus Receptor/PVR matched pair antibodies Capture: HA723464, Human Poliovirus Receptor/PVR Rabbit mAb [PSH12-54] Detector: HA723465, Human Poliovirus Receptor/PVRn Rabbit mAb [PSH12-55] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723464) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Poliovirus Receptor/PVRn protein (HA210957) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723465, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native PVR in human samples. Capture: HA723464, Human Poliovirus Receptor/PVR Rabbit mAb [PSH12-54] Detector: HA723465, Human Poliovirus Receptor/PVRn Rabbit mAb [PSH12-55] Interpolated concentration of native PVR was measured in duplicate at different sample concentrations. Undiluted samples were 1% human serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean PVR concentration was determined to be 133,172 pg/mL in human serum. |
|
Fig3:
Interpolated concentrations of native PVR in U-87 MG and Raji extract samples based on a 1000 µg/ml extract load. Capture: HA723464, Human Poliovirus Receptor/PVR Rabbit mAb [PSH12-54] Detector: HA723465, Human Poliovirus Receptor/PVRn Rabbit mAb [PSH12-55] Interpolated concentration of native PVR was measured in duplicate at different sample concentrations and interpolated from the PVR standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean PVR concentration was determined to be 7,903 pg/mL in U-87 MG cell extract. There was no detectable signal in Raji cell extract. |
|
Fig4:
Interpolated concentrations of spiked PVR in cell culture media samples. Capture: HA723464, Human Poliovirus Receptor/PVR Rabbit mAb [PSH12-54] Detector: HA723465, Human Poliovirus Receptor/PVRn Rabbit mAb [PSH12-55] The concentrations of PVR were measured in duplicates, interpolated from the PVR standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.