Biotin Conjugated Human Alpha-Synuclein Recombinant Rabbit Monoclonal Antibody [PSH12-56]
cat.: HA723469B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH12-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Alpha-Synuclein aa 1-140. |
| Positive control: | Recombinant Human Alpha-Synuclein protein (HA211150). |
| Subcellular location: | Cytoplasm, Membrane, Nucleus, Synapse, Secreted, Cell projection, axon. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [JB42-33] to Human Alpha-Synuclein antibody (Capture) (HA723467) and Recombinant Human Alpha-Synuclein protein (HA211150) as the standard. The reference range value is 313-20,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P37840 Human |
| Alternative names: | Alpha synuclein Alpha-synuclein Alpha-synuclein, isoform NACP140 alphaSYN MGC105443 MGC110988 MGC127560 MGC64356 NACP Non A beta component of AD amyloid Non A4 component of amyloid Non A4 component of amyloid precursor Non-A beta component of AD amyloid Non-A-beta component of alzheimers disease amyloid , precursor of Non-A4 component of amyloid precursor Non-A4 component of amyloid, precursor of OTTHUMP00000218549 OTTHUMP00000218551 OTTHUMP00000218552 OTTHUMP00000218553 OTTHUMP00000218554 PARK 1 PARK 4 PARK1 PARK4 Parkinson disease (autosomal dominant, Lewy body) 4 Parkinson disease familial 1 SNCA Snca synuclein Snca synuclein, alpha (non A4 component of amyloid precursor) SYN Synuclein alpha Synuclein alpha 140 Synuclein, alpha (non A4 component of amyloid precursor) SYUA_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Human Alpha-Synuclein matched pair antibodies Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33] Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723467) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human Alpha-synuclein protein (HA211150) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723468, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native alpha-Synuclein in SH-SY5Y extract samples based on a 1,000 µg/ml extract load. Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33] Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56] The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-Synuclein concentration was determined to be 2,195 pg/ml in SH-SY5Y extract. |
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Fig3:
Interpolated concentrations of native alpha-Synuclein in human serum samples. Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33] Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56] The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curve and corrected for sample dilution. Undiluted samples are human serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-Synuclein concentration was determined to be 21.2 ng/ml in human serum. |
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Fig4:
Interpolated concentrations of spiked alpha-Synuclein in human cell culture media samples. Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33] Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56] The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.