Human Nectin-4 Recombinant Rabbit Monoclonal Antibody [PSH12-57]
cat.: HA723470
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-57 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Nectin-4 aa 32-349 (HA210883). |
| Positive control: | Recombinant Human Nectin-4 protein (HA210883). |
| Subcellular location: | Cell membrane Curated, Cell junction, adherens junction, Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-58] to Human Nectin-4 antibody (Detector) (HA723471) and Recombinant Human Nectin-4 protein (HA210883) as the standard. The reference range value is 39.1-5,000 pg/mL. |
| Uniprot #: | SwissProt: Q96NY8 Human |
| Alternative names: | DKFZp686K05193 EDSS1 Ig superfamily receptor LNIR Nectin 4 Nectin-4 poliovirus receptor-related 4 Processed poliovirus receptor-related protein 4 PRR4 pvrl4 PVRL4_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of human Nectin-4 matched pair antibodies Capture: HA723470, Human Nectin-4 Rabbit mAb [PSH12-57] Detector: HA723471, Human Nectin-4 Rabbit mAb [PSH12-58] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723470) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Nectin-4 protein (HA210883) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723471, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Nectin-4 in MCF7 and MDA-MB-231 extract samples based on a 1,000 µg/ml extract load. Capture: HA723470, Human Nectin-4 Rabbit mAb [PSH12-57] Detector: HA723471, Human Nectin-4 Rabbit mAb [PSH12-58] The concentrations of Nectin-4 were measured in triplicates, interpolated from the Nectin-4 standard curve and corrected for sample dilution. Undiluted samples are MCF7 extract 100% and MDA-MB-231 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean Nectin-4 concentration was determined to be 1,829 pg/ml in MCF7 extract and undetectable in MDA-MB-231 extract. |
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Fig3:
Interpolated concentrations of native Nectin-4 in human urine samples. Capture: HA723470, Human Nectin-4 Rabbit mAb [PSH12-57] Detector: HA723471, Human Nectin-4 Rabbit mAb [PSH12-58] The concentrations of Nectin-4 were interpolated from the Nectin-4 standard curve and corrected for sample dilution. Undiluted samples are human urine 50%. The mean Nectin-4 concentration was determined to be 3,260 pg/ml in human urine. |
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Fig4:
Interpolated concentrations of spiked Nectin-4 in human cell culture media samples. Capture: HA723470, Human Nectin-4 Rabbit mAb [PSH12-57] Detector: HA723471, Human Nectin-4 Rabbit mAb [PSH12-58] The concentrations of Nectin-4 were measured in duplicates, interpolated from the Nectin-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.