Human CSF-1-R Recombinant Rabbit Monoclonal Antibody [PSH12-66]
cat.: HA723474
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: ELISA(Cap), ELISA(Det)
Clonality: Monoclonal
Clone number: PSH12-66
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Recombinant protein within Human CSF-1-R aa 20-517 (HA211149).
Positive control: Recombinant Human CSF-1-R protein (HA211149).
Subcellular location: Cell membrane.
Recommended Dilutions:
  ELISA(Cap)
  ELISA(Det)
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-67] to Human CSF-1-R antibody (Detector) (HA723475) and recombinant Human CSF-1-R (HA211149) protein as the standard. The reference range value is 39.1-5000 pg/mL.
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-68] to Human CSF-1-R antibody (Capture) (HA723477) and recombinant Human CSF-1-R protein (HA211149) as the standard. The reference range value is 156-5,000 pg/mL.
Uniprot #: SwissProt: P07333 Human
Alternative names: C FMS CD 115 CD115 CD115 antigen CFMS Colony stimulating factor 1 receptor Colony stimulating factor I receptor CSF 1 R CSF 1R CSF-1 receptor CSF-1-R CSF1 R CSF1R CSF1R_HUMAN CSFR EC 2.7.10.1 FIM 2 FIM2 FMS FMS proto oncogene FMS protooncogene HDLS M-CSF Receptor M-CSF-R Macrophage colony stimulating factor 1 receptor Macrophage colony stimulating factor I receptor Macrophage colony-stimulating factor 1 receptor McDonough feline sarcoma viral (v fms) oncogene homolog MCSFR Oncogen FMS Proto-oncogene c-Fms V-FMS McDonough feline sarcoma viral oncogen homolog, formerly
Images
HA723474_1.png Fig1: Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies

Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66]
Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723474) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSF-1-R protein (HA211149) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723475, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723474_2.png Fig2: Interpolated concentrations of native CSF1R in THP-1 and Raji cell extract samples based on a 1000 µg/ml extract load.

Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66]
Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67]

Interpolated concentration of native CSF1R was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CSF1R concentration was determined to be 3144 pg/mL in THP-1 cell extract and undetectable in Raji cell extract.
HA723474_3.png Fig3: Interpolated concentrations of spiked CSF1R in cell culture media samples.

Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66]
Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67]

The concentrations of CSF1R were measured in duplicates, interpolated from the CSF1R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
HA723474_4.png Fig4: Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies

Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68]
Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723477) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSF-1-R protein (HA211149) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723474, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA723474_5.png Fig5: Interpolated concentrations of native CSF1R in THP-1 and Raji cell extract samples based on a 1000 µg/ml extract load.

Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68]
Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66]

Interpolated concentration of native CSF1R was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CSF1R concentration was determined to be 3782 pg/mL in THP-1 cell extract and undetectable in Raji cell extract.
HA723474_6.png Fig6: Interpolated concentrations of spiked CSF1R in cell culture media samples.

Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68]
Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66]

The concentrations of CSF1R were measured in duplicates, interpolated from the CSF1R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.