HRP Conjugated Human CSF-1-R Recombinant Rabbit Monoclonal Antibody [PSH12-66]
cat.: HA723478H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH12-66 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CSF-1-R aa 20-517 (HA211149). |
| Positive control: | Recombinant Human CSF-1-R protein (HA211149). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-66] to Human CSF-1-R antibody (Detector) (HA723474) and recombinant Human CSF-1-R protein (HA211149) as the standard. The reference range value is 1,370-111,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P07333 Human |
| Alternative names: | C FMS CD 115 CD115 CD115 antigen CFMS Colony stimulating factor 1 receptor Colony stimulating factor I receptor CSF 1 R CSF 1R CSF-1 receptor CSF-1-R CSF1 R CSF1R CSF1R_HUMAN CSFR EC 2.7.10.1 FIM 2 FIM2 FMS FMS proto oncogene FMS protooncogene HDLS M-CSF Receptor M-CSF-R Macrophage colony stimulating factor 1 receptor Macrophage colony stimulating factor I receptor Macrophage colony-stimulating factor 1 receptor McDonough feline sarcoma viral (v fms) oncogene homolog MCSFR Oncogen FMS Proto-oncogene c-Fms V-FMS McDonough feline sarcoma viral oncogen homolog, formerly |
Images
|
Fig1:
Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68] Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723477) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSF-1-R protein (HA211149) starting from 111,000 pg/ml to 0 pg/ml and detect antibody (HA723474, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.