Livin Recombinant Rabbit Monoclonal Antibody [PSH12-72]
cat.: HA723483
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH12-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Livin aa 1-250. |
| Positive control: | SK-MEL-28 cell lysate, human breast cancer tissue, human placenta tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Golgi apparatus |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q96CA5 Human |
| Alternative names: | Baculoviral IAP repeat containing 7 Baculoviral IAP repeat containing protein 7 Baculoviral IAP repeat-containing protein 7 BIRC 7 birc7 BIRC7_HUMAN KIAP Kidney inhibitor of apoptosis protein Livin Livin inhibitor of apotosis Melanoma inhibitor of apoptosis protein ML IAP ML-IAP MLIAP RING finger protein 50 RNF 50 RNF50 |
Images
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Fig1:
Western blot analysis of Livin on different lysates with Rabbit anti-Livin antibody (HA723483) at 1/5,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723483) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Livin antibody (HA723483) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723483) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Livin antibody (HA723483) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723483) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Livin was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with HA723483 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723483 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SK-MEL-28 cell lysate (input) Lane 2: HA723483 IP in SK-MEL-28 cell lysate Lane 3: Rabbit IgG instead of HA723483 in SK-MEL-28 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 12 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.